Target Enrichment | Gene Sequencing | Kailos Genetics

targetrich

Targeted Enrichment

with a fast, scalable single-tube workflow

TargetRich™ custom designs provide the coverage critical to your research success, enabling you to effectively target the regions you desire, while delivering the results you need.  Using a technology that can simultaneously select many target regions across multiple samples, TargetRich maximizes throughput capacity for next generation sequencing.

TargetRich achieves this high level of specificity through two rounds of target-specific targeting, employing clean-up steps to remove any off-target products. This high level of specificity enables TargetRich to efficiently use sequencer capacity and delivers results at a low cost per targeted base pair.

Fast, scalable and easy to use with just 1.25 hours of hands on time, TargetRich requires just 10ng of starting material to enrich your research samples.

Features

  • High Primary Design Coverage
  • High Alignment to Targeted Regions
  • High Percentage of On-target Sequence Data and Focused Content to Achieve High Read Depths
  • Single Tube – Start to Finish – Addition Only Workflow
  • Just 1.25 Hours Hands-On Time
  • Low DNA Input Requirement

Targeted Enrichment

TargetRich was based on Nested PatchPCR™, a proprietary technology developed to enrich genomic regions of interest prior to next generation sequencing.

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For life science research only. Not for use in diagnostic procedures.

Custom panel designs are available through Kailos Genetics.

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TargetRich primary design for an undisclosed party comprised 158 segments, direct amplicon. A tiling approach was used. Three samples run in duplicate were sequenced on a single Illumina MiSeq (2×150) sequencer run.

The Results
Result Sample P-1 P-100
1200 C-507.S1 99.47% 90.82%
1201 C-507.S1 99.03% 91.78%
1202 C-510.S1 99.72% 95.28%
1203 C-510.S1 99.20% 88.73%
1204 C-511.S1 99.74% 92.75%
1205 C-511.S1 99.20% 91.13%

TargetRich Design Delivered

  • Gene 1 (24 Exons): 100% Coverage, except for 50% of First Exon
  • Gene 2 (27 Exons): 100% Coverage, except 10% of the First Exon, 13% of Exon 17, and 2% of Exon 27
  • High Alignment to Targeted Region: 95.8%
  • Read Depth > 100 for 91.7% of Bases

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TargetRich™ CR Survey for Illumina – Targeted enrichment of 62 genes frequently mutated and studied in cancer research.

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Cancer Research CRX v1.10 Content
Gene Number of Amplicons Base-pairs Covered
BRAF 17 4,074
EGFR 24 4,521
FLT3 22 4,683
JAK2 22 6,233
KIT 20 4,375
KRAS 5 1,249
PIK3CA 15 4,519
PTEN 9 3,062
TP53 8 1,596
VEGFA 5 568
Total: 147 34,880

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